I was pulling apart an old LaserTec C140 confocal microscope. Here are some pics! Here’s the main optical block:
There’s a photodiode, I assume with a pinhole and CCD sensor here:
On the other side of the unit there’s an FPGA.
Under the unit there’s a stepper (right) and a BL55 “Laserscale”.
I’ve been trying to figure out how all this fits together. The unit appears to be white light only, but I’m unclear on where the actuation is. It looks like everything is on the bottom of the unit. I guess the BL55 laser scale is used in combination with that stepper. But I’m not clear if there’s a full sub-micron precision XYZ stage here or if there’s something else going on. I was really expecting to see Galvos to scan the spot across the sample… hmmm.
I was trying to interface directly with the I2C on the Arducam Pivistation 5 Klarity camera module for… reasons.
Anyway I figured this would just expose the I2C on the IMX283… but it doesn’t seem to. There seems in fact to be another little microcontroller (GD e230f8?) there which uses a different I2C command set. It seems similar to the interface from this camera:
Anyway… I was able to get some info out of it using a Glasgow Interface Explorer. This note is here so someone else doesn’t waste time wondering why the IMX283 (which I think should respond on 0x1a) is responding on 0x0c on this camera.
The Glasgow commands required are below:
Also, it seems that you can crash the microcontroller pretty easily by doing additional reads?
“If we recall that an Ampere corresponds to a charge flow of 1 Coulomb each second and further, use the fact that the charge on a monovalent ion is approximately 1.6 x 10-19 Coulombs (that is 1 electron or 1 proton charge), then we see that a current of about one pA corresponds to roughly 107 ions passing through the channel each second. This value is in agreement with measurements” http://book.bionumbers.org/how-many-ions-pass-through-an-ion-channel-per-second/
Double stranded Lambda DNA (16um long, 48kb). Translocates in 3 different confirmations. Grabbed at the end. Grabbed in the middle (completely folded), Grabbed near an edge (partly folded). Difference in current levels is ~1nS between each confirmation (200pA). 22nm pore is used. Translocation time ~3ms.
My name is Nava Whiteford. I’ve worked for a few sequencing companies. I have equity in a few sequencing companies based on my previous employment (I try to be unbiased in my posts). You can contact me at: [email protected]
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