{"id":7052,"date":"2023-09-14T04:23:23","date_gmt":"2023-09-14T04:23:23","guid":{"rendered":"https:\/\/41j.com\/blog\/?p=7052"},"modified":"2023-09-14T04:24:03","modified_gmt":"2023-09-14T04:24:03","slug":"pacific-biosciences-accuracy-notes","status":"publish","type":"post","link":"https:\/\/41j.com\/blog\/2023\/09\/pacific-biosciences-accuracy-notes\/","title":{"rendered":"Pacific Biosciences Accuracy Notes"},"content":{"rendered":"\n<figure class=\"wp-block-table\"><table><tbody><tr><td><\/td><td>Date<\/td><td>Overall<\/td><td>Insertions<\/td><td>Deletions<\/td><td>Substitutions<\/td><td>Read Length<\/td><td>Ref<\/td><\/tr><tr><td>PacBio RS II<\/td><td>~2013<\/td><td>~11%<\/td><td>\u201cpredominantly indel\u201d<\/td><td>\u201cpredominantly indel\u201d<\/td><td>\u201cpredominantly indel\u201d<\/td><td><\/td><td><a href=\"http:\/\/www.pacb.com\/wp-content\/uploads\/2015\/09\/Perspective_UnderstandingAccuracySMRTSequencing1.pdf\"><u>Vendor<\/u><\/a><\/td><\/tr><tr><td>PacBio RS II<\/td><td>2020<\/td><td>~13%<\/td><td>8%<\/td><td>3%<\/td><td>1.68%<\/td><td><\/td><td><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7671305\/\"><u>Academic Benchmark<\/u><\/a><\/td><\/tr><tr><td>PacBio RS II CLR<\/td><td>2020<\/td><td>12.88%<\/td><td>8.04%<\/td><td>3.16%<\/td><td>1.68%<\/td><td>8899<\/td><td><a href=\"https:\/\/academic.oup.com\/nargab\/article\/2\/2\/lqaa037\/5843804\"><u>Academic Benchmark<\/u><\/a><\/td><\/tr><tr><td>PacBio RS II Subread<\/td><td>2017<\/td><td>14.2%<\/td><td>5.92%<\/td><td>3.01%<\/td><td>5.27%<\/td><td><\/td><td><a href=\"https:\/\/f1000research.com\/articles\/6-100\/v2\"><u>Academic Benchmark<\/u><\/a><\/td><\/tr><tr><td>PacBio Sequel I CLR<\/td><td>2019<\/td><td>12%<\/td><td><\/td><td><\/td><td><\/td><td><\/td><td><a href=\"https:\/\/get.genotoul.fr\/wp-content\/uploads\/2019\/10\/INRA_Toulouse_HiFi_final.pdf\"><u>Inferred from Vendor Claim<\/u><\/a><\/td><\/tr><tr><td>PacBio Sequel II CLR<\/td><td>2019<\/td><td>10%<\/td><td><\/td><td><\/td><td><\/td><td>~50Kb<\/td><td><a href=\"https:\/\/get.genotoul.fr\/wp-content\/uploads\/2019\/10\/INRA_Toulouse_HiFi_final.pdf\"><u>Inferred from Vendor Claim<\/u><\/a><\/td><\/tr><tr><td>Revio (Subread)<\/td><td>2023<\/td><td>10%<\/td><td><\/td><td><\/td><td><\/td><td>All: 100Kb+?<\/td><td>Inferred from Vendor Claim<\/td><\/tr><tr><td>PacBio RS II CCS<\/td><td>2017<\/td><td>1.72<\/td><td>0.087%<\/td><td>0.34%<\/td><td>1.3%<\/td><td>1772<\/td><td><a href=\"https:\/\/f1000research.com\/articles\/6-100\/v2\"><u>Academic Benchmark<\/u><\/a><\/td><\/tr><tr><td>PacBio Sequel HiFi<\/td><td>2022<\/td><td>0.25%<\/td><td>0.1%<\/td><td>0.13%<\/td><td>0.02%<\/td><td><\/td><td><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9713900\/\"><u>Academic Note<\/u><\/a><\/td><\/tr><tr><td>PacBio Sequel II CCS<\/td><td>2019<\/td><td>0.14%<\/td><td><\/td><td><\/td><td><\/td><td><\/td><td><a href=\"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01368-19\"><u>Academic Benchmark<\/u><\/a><\/td><\/tr><tr><td>PacBio Sequel&nbsp;&nbsp;II HiFi<\/td><td>2019<\/td><td>&lt;0.1%<\/td><td><\/td><td><\/td><td><\/td><td>20Kb<\/td><td><a href=\"https:\/\/get.genotoul.fr\/wp-content\/uploads\/2019\/10\/INRA_Toulouse_HiFi_final.pdf\"><u>Vendor Claim<\/u><\/a><\/td><\/tr><tr><td>Revio (All HiFi)<\/td><td>2023<\/td><td>&lt;0.1%<\/td><td><\/td><td><\/td><td><\/td><td>16.5Kb<\/td><td>Vendor Claim<\/td><\/tr><\/tbody><\/table><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Date Overall Insertions Deletions Substitutions Read Length Ref PacBio RS II ~2013 ~11% \u201cpredominantly indel\u201d \u201cpredominantly indel\u201d \u201cpredominantly indel\u201d Vendor PacBio RS II 2020 ~13% 8% 3% 1.68% Academic Benchmark PacBio RS II CLR 2020 12.88% 8.04% 3.16% 1.68% 8899 Academic Benchmark PacBio RS II Subread 2017 14.2% 5.92% 3.01% 5.27% Academic Benchmark PacBio Sequel [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[],"class_list":["post-7052","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_shortlink":"https:\/\/wp.me\/p1RRoU-1PK","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts\/7052","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/comments?post=7052"}],"version-history":[{"count":1,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts\/7052\/revisions"}],"predecessor-version":[{"id":7053,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts\/7052\/revisions\/7053"}],"wp:attachment":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/media?parent=7052"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/categories?post=7052"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/tags?post=7052"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}