{"id":6340,"date":"2021-11-16T01:58:45","date_gmt":"2021-11-16T01:58:45","guid":{"rendered":"https:\/\/41j.com\/blog\/?p=6340"},"modified":"2021-11-16T12:12:14","modified_gmt":"2021-11-16T12:12:14","slug":"nanodrop-notes","status":"publish","type":"post","link":"https:\/\/41j.com\/blog\/2021\/11\/nanodrop-notes\/","title":{"rendered":"Nanodrop Notes"},"content":{"rendered":"\n

I recently wrote about various UV-Vis spectrophotometers on the substack<\/a>. As part of this I discussed the Nanodrop and how its architecture differs from other instruments and the advantages this gives.<\/p>\n\n\n\n

<\/p>\n\n\n\n

The CCD is quite possibly a Sony ILX511<\/a> this sensor was a popular CCD in a number of spectrometers, I’ve previously designed a small interface<\/a> for this sensor.<\/p>\n\n\n\n

The layout seems pretty simple, the light from the fiber comes in, hits a mirror then is reflected off a diffraction grating and heads to the CCD:<\/p>\n\n\n\n

\"\"<\/figure><\/div>\n\n\n\n

The auction contains a number of other photos, which includes a fiber cable. I assume this fiber (and all the other components) must be UV compatible which will increase the cost. But overall, its a very simple optical system.<\/p>\n\n\n\n

Thermo documentation suggests that older Nanodrops use Xenon Flash lamps<\/a>. These give a relatively broad output. It doesn’t seem like the Nanodrop has any kind of sensor on the output of the lamp (unlike more traditional UV-Vis instruments) this will make taking a blank measurement all the more important. The blank will be compensating for both emission differences across wavelength in the lamp and the absorbance of the buffer.<\/p>\n\n\n\n

Newer Nanodrops appear to use LEDs, I assume this must be a combination of UV and visible LEDs to cover the entire spectrum.<\/p>\n\n\n\n

Other references<\/a> show the Nanodrop setup, and suggest that fused silica (quartz) is used:<\/p>\n\n\n\n

\"\"<\/figure><\/div>\n\n\n\n

The Nanodrop patents<\/a> are pretty informative, and make it reasonably clear that the fiber optical “pedestals” are just the ends of a couple of SMA connectors “typically the end of an industry standard SMA fiber optic connector\u00a010<\/strong>, FIG. 3a\u00a0<\/em>(found as connectors on the ends of optical patch cords like p\/n P-400-2-UV-VIS from Ocean Optics inc. of Dunedin, Fla.)… For most SMA connectors the approximate 2 mm end diameter can be effectively covered with 2 microliters of water or a water-based solutions.”<\/p>\n\n\n\n

\"\"<\/figure><\/div>\n\n\n\n

A few other pictures from the auction<\/a> are below, while the auction is listed as Nanodrop monochromator. I suspect this may however be mislabeled, and am continuing to search for Nanodrop teardown images…<\/p>\n\n\n\n

\"\"<\/figure><\/div>\n\n\n\n
\"\"<\/figure><\/div>\n\n\n\n
\"\"<\/figure><\/div>\n","protected":false},"excerpt":{"rendered":"

I recently wrote about various UV-Vis spectrophotometers on the substack. As part of this I discussed the Nanodrop and how its architecture differs from other instruments and the advantages this gives. The CCD is quite possibly a Sony ILX511 this sensor was a popular CCD in a number of spectrometers, I’ve previously designed a small […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[],"class_list":["post-6340","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_shortlink":"https:\/\/wp.me\/p1RRoU-1Eg","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts\/6340","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/comments?post=6340"}],"version-history":[{"count":5,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts\/6340\/revisions"}],"predecessor-version":[{"id":6357,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts\/6340\/revisions\/6357"}],"wp:attachment":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/media?parent=6340"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/categories?post=6340"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/tags?post=6340"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}