{"id":3025,"date":"2015-10-22T18:12:23","date_gmt":"2015-10-22T18:12:23","guid":{"rendered":"http:\/\/41j.com\/blog\/?p=3025"},"modified":"2015-10-22T18:12:23","modified_gmt":"2015-10-22T18:12:23","slug":"axopatch-200a-with-micropipette-first-tests","status":"publish","type":"post","link":"https:\/\/41j.com\/blog\/2015\/10\/axopatch-200a-with-micropipette-first-tests\/","title":{"rendered":"Axopatch 200A with micropipette first tests"},"content":{"rendered":"<p><iframe loading=\"lazy\" width=\"560\" height=\"315\" src=\"https:\/\/www.youtube.com\/embed\/NkZRvCmbR7o\" frameborder=\"0\" allowfullscreen><\/iframe><\/p>\n<p>The video above shows my test tentative experiments using the Axopatch 200A and a micropipette pulled using the Sutter P-97 as I <a href=\"http:\/\/41j.com\/blog\/2015\/10\/playing-with-a-micropipette-puller-sutter-p-97\/\">described yesterday<\/a>.<\/p>\n<p>I used KCL diluted to ~100mM and sucked the solution into the pipette using a syringe. I didn&#8217;t have the correct tubing and pulled the sheath off a piece of silicon sheathed wire. It seemed to work ok. Drawing fluid into the pipette wasn&#8217;t easy I think I either have the wrong clamp for the pipette or perhaps it needs some PTFE tape to seal it.<\/p>\n<p>Anyway, it appeared to more or less work and I could vary the current through the solution by changing the bias voltage. This is all I wanted for a first test and I now have a short list of bits and pieces I&#8217;d need to acquire to go further.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The video above shows my test tentative experiments using the Axopatch 200A and a micropipette pulled using the Sutter P-97 as I described yesterday. I used KCL diluted to ~100mM and sucked the solution into the pipette using a syringe. I didn&#8217;t have the correct tubing and pulled the sheath off a piece of silicon [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[],"class_list":["post-3025","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_shortlink":"https:\/\/wp.me\/p1RRoU-MN","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts\/3025","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/comments?post=3025"}],"version-history":[{"count":1,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts\/3025\/revisions"}],"predecessor-version":[{"id":3026,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/posts\/3025\/revisions\/3026"}],"wp:attachment":[{"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/media?parent=3025"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/categories?post=3025"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/41j.com\/blog\/wp-json\/wp\/v2\/tags?post=3025"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}